National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bovine Leukocyte Adhesion Deficiency (BLAD), Bovine Citrullinaemia (BC), and Complex Vertebral Malformation (CVM) are the common autosomal recessive disorders in cattle affecting dairy industry. The present study was designed to standardize allele-specific PCR based genotyping method for examining the occurrence of these disorders in Indian cattle population. A total of 327 indigenous (Bos indicus), exotic (HF) cattle breeds and HF crossbreds (Bos taurus × Bos indicus) from various parts of India were screened. An allele specific PCR technique was applied to detect mutation in specific DNA target sequences. The amplified products were purified and sequenced to confirm the change in nucleotide in the selected regions. Allele-specific PCR-based screening methodology was optimized for genotyping. No animals were found as carrier of these genetic abnormalities in the present study. Allele-specific PCR-based screening methodology may be efficiently employed to screen the animal population for these genetic defects. All tested animals were free from the aforementioned genetic disorders. But this may not provide us conclusively cattle herds maintained at the study area are free from these genetic disorders. Therefore, it is highly recommended to continue screening procedure to check undesirable transmission mutated version of alleles in Indian cattle population.
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