International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 2975-2987

Biodiesel Production by Pichia occidentalis MH879824.1
Laila Ramadan, Yasser Fathy Abdelaliem* and Salem Abdelfattah Mahfoz
Department of Agricultural Microbiology, Faculty of Agricultural, Fayoum University, Egypt
*Corresponding author

A lipid producing isolate (LPI 65) was identified by a molecular genetics technique as Pichia occidentalis MH879824.1 was investigated for its ability to produce biodiesel. The isolate was screened for its ability to accumulate lipids within the cells by culturing on nitrogen-limited medium (NLM) and using Sudan black B staining technique. Estimation of lipid content % was performed. The effect of different glucose concentrations in NLM on dry biomass and lipid weight were conducted and results showed that the best concentration of glucose was 70 g/L. The possibility of using different wastes such as sugar cane molasses, waste food oil and shortening, whey permeate, and waste motor oil as carbon sources for lipid production were determined, and the results showed that the best waste for lipid production was waste motor oil. Optimization of fermentation conditions such as effect of NLM composition, NLM pH, incubation temperature, and incubation period were also investigated and results of these tests showed that the best lipid accumulation when NLM5, at pH 5.5 and incubated at 28oC for 120 h. Fatty acids profile analysis achieved that the lipids extracted from the isolate mainly contained the principal fatty acids (triacylglycerol’s, TAGs) which are similar to that of vegetable oils mainly oleic, linoleic and palmitic acid.

Keywords: Honey Oleaginous yeasts, Biodiesel, lipid extraction, Fatty acids analysis, 26S rDNA gene, Nitrogen-limited medium

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How to cite this article:

Laila Ramadan, Yasser Fathy Abdelaliem and Salem Abdelfattah Mahfoz. 2019. Biodiesel Production by Pichia occidentalis MH879824.1.Int.J.Curr.Microbiol.App.Sci. 8(4): 2975-2987. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.