International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 2686-2693

Assessment of Genetic Variability among the Landraces of Little Millets Panicum sumatrense from Different District of Madhya Pradesh
Lalit Prashad Singh Rajput1, Keerti Tantwai1*, Sajjan Kumar Pooniya1 and Koji Tsuji2
1Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur - 482 004, Madhya Pradesh, India
2Faculty and Graduate School of Education, Chiba University, Chiba – 263-8522, Japan
*Corresponding author

Present study was conducted on genetic diversity using ISSR markers for a total of 40 landraces of little millet (Panicum sumatrense) collected from five different districts of Madhya Pradesh. Ten ISSR markers amplified total 42 loci while 32 loci showed 76.19% polymorphism. Maximum number (06) of alleles were scored by the primers UBC-807 whereas, minimum number of alleles (03) were scored by the primers UBC-816. Percentage of the number of polymorphic loci within population among the three regions, the highest frequency of polymorphism was found in the Dindori region (97.61) followed by the Betul region (80.95) and the lowest were in the Chhindwara region (40.47). Cluster analysis was estimated and a dendrogram was generated using Unweighted Pair Group Analysis (UPGMA). The highest genetic variability was observed between Amwa-38, Shivri-31 and Khaparipani-24 collected from Rewa and Dindori both of them grouped distantly. The highest PIC value (0.53) was observed by using primer UBC-853 having 06 alleles among the 40 landraces of little millets. The results indicated that ISSR marker system can be effectively used in determination of genetic relationship necessary for their conservation and breeding programs among the landraces of little millets grown in different districts of Madhya Pradesh, India.

Keywords: Polymorphism, UPGMA, Landraces, Genetic variability, Genetic conservation, Phylogenetic relationship

Download this article as Download

How to cite this article:

Lalit Prashad Singh Rajput, Keerti Tantwai, Sajjan Kumar Pooniya and Koji Tsuji. 2019. Assessment of Genetic Variability among the Landraces of Little Millets Panicum sumatrense from Different District of Madhya Pradesh.Int.J.Curr.Microbiol.App.Sci. 8(4): 2686-2693. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.