International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1929-1939

Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus from the Retail Meat Shops and Customers
Asima Zehra*, Maliha Gulzar, Randhir Singh and Simranpreet Kaur
1School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana-141004, Punjab, India
*Corresponding author

This study was completed to know the prevalence of Methicillin-Susceptible (MSSA) and Methicillin-Resistant S. aureus (MRSA) in swab samples from retail meat shops (RM) and customers (CU) in five districts of Punjab, India. An aggregate of 182 swabs samples was aseptically collected from RM shops and customers. The collected samples were processed for an isolation of S. aureus isolates. The phenotypic resistance of S. aureus isolates was most noteworthy to Penicillin (PEN, 97.83%) trailed by Ciprofloxacin (CPH, 56.52%), Tetracycline (TET, 36.96%), Trimethoprim-Sulfamethoxazole (TSH, 34.78%) and Erythromycin (ERY, 17.39%). However, low resistance was observed to Clindamycin, Chloramphenicol, Oxacillin, Ceftriaxone, and that fluctuated from 2%-7%. None of the isolates was phenotypically resistant to vancomycin (MIC 0.5-2 µg/ml). A large portion of S. aureus isolates (58.69%, 95% CI 43.63-61.93) were Multi-drug resistant (MDR) and carried resistant genes to penicillin (blaZ), oxacillin (mecA), gentamicin (aacA-aphD), erythromycin (ermB, ermC) and tetracycline (tetK, tetM). Two S. aureus isolates were borderline oxacillin resistant (BORSA) with MIC 4 µg/ml and one isolate was MRSA (Oxacillin MIC 16 µg/ml) with a genotypic profile, mecA+blaZ+aacA-aphD+tetK+ermC+. Among the erythromycin-resistant or intermediate resistant isolates, none expressed inducible macrolide lincosamide and streptogramin (MLSB) phenotype (ERY+/CLI-, D+) except for one MSSA isolates from CU hand swab sample that demonstrated a constitutive MLSB phenotype (Erm+/Cli+, D-).

Keywords: Staphylococcus aureus, Multidrug resistance, Epsilometer test, MRSA, BORSA, Swab

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Asima Zehra, Maliha Gulzar, Randhir Singh and Simranpreet Kaur. 2019. Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus from the Retail Meat Shops and Customers.Int.J.Curr.Microbiol.App.Sci. 8(4): 1929-1939. doi:
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