International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1844-1851

Effect of Photoperiod on In vitro Culture of Guggul [Commiphora wightii (Arnott)] – A Medicinal Plant
Rajani Verma1*, M.L. Jakhar2 and Ravi Kumar1
Department of Plant Breeding and Genetics, SKN College of Agriculture, Jobner, India
*Corresponding author

Present investigation was carried out the effect of different photoperiod regimes on shoot bud induction, callus induction and shoot regeneration from callus culture in leaf explants of guggul. Standard protocols micropropagation protocol (1.5 mg/l BAP) for nodal segment and (2.0 mg/l Kn) for shoot apex explant and callus induction (2.0 mg/l 2,4-D) and regeneration protocol (1.5 mg/l Kn+ 1.0 mg/l 2,4-D)] were subjected to different photoperiod regimes (16:8, 14:10, 12:12 and 8:16). The cultures were incubated at 25±2°C with a light intensity of 3000 lux. 14:10 hours photoperiod regime was found best for shoot bud induction, callus differentiation and de novo shoot development among all the tested photoperiod regimes.

Keywords: Nodal segment, Shoot apex, Leaf, Photoperiod, Callus, in vitro, Guggul.

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How to cite this article:

Rajani Verma, M.L. Jakhar and Ravi Kumar. 2019. Effect of Photoperiod on In vitro Culture of Guggul [Commiphora wightii (Arnott)] – A Medicinal Plant.Int.J.Curr.Microbiol.App.Sci. 8(4): 1844-1851. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.