National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present study was undertaken to assess the hygiene quality of the meat, milk and also its prevalence on human population. A total of 330 samples including, chicken meat (n=98), Chevon (n=82), raw milk samples (n=90) and human urine (n=56) and stool samples (n=4) Samples were aseptically collected from different districts of Chhattisgarh. Samples were processed for isolation of Salmonella further subjected for the different biochemical tests and confirmed by serotyping. Confirmed isolates were tested for its antibiogram pattern against 13 different antibiotic discs. Isolates were examined for phenotypic method for Extended Spectrum β-lactamases (ESBLs) by double disc synergy test and molecular characterization was done by multiplex PCR method. A total of 14.2% (47/330) samples were positive for Salmonella spp. highest prevalence rate of 27.55% (27/98) were observed in chicken meat, followed by 15% (9/60) and 13.41% (11/82) samples were found positive for Salmonella in contrary none of the milk samples found positive for Salmonella. Serotyping reveals the presence of S. typhiurium and S. enteritidis serotype among the isolates. Isolates showed highest sensitivity (95.7%) against imipenem and however 89.2 % isolates were found resistant to oxytetracylin. Out of 47 isolates 2.12% isolates were found as ESBL positive by phenotypic and genotypic method.
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