International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 2339-2346

Evaluation of Diagnostic Test in Emerging Carbapenem Resistant Gram Negative Bacilli in Patients admitted to Tertiary Care Centre in North India
Munesh Kumar Sharma1*, Dakshina Bisht1 and Shekhar Pal2
1Department of Microbiology, Santosh Medical College, Ghaziabad, NCR Delhi, India
2Department of Microbiology, Doon Medical College, Dehradun, India
*Corresponding author

Carbapenem antibiotics are very often used against multidrug resistant strains clinically troublesome pathogens which developed and proved that the resistance and metallo-β-lactamases (MBL) production were a disaster in treating infections. The identification and detection of MBL-producing bacterial strains were having crucial importance for the prevention of nosocomial infections. Therefore the present study was undertaken for screening MBL production Gram Negative bacteria. One hundred twenty two 122 consecutive Non-repetitive isolates of gram negative bacilli clinical isolates were subjected to susceptibility testing by disc-diffusion test on Mueller Hinton Agar. Meropenem resistant (MR) strains MBL production among MR stains were further screened by Meropenem- EDTA combined disc synergy test (M-CDST) and Meropenem-EDTA double-disc synergy test (M-DDST). A total of 31 isolates showed resistance to Meropenem which were screened and 29 (93.55%) isolates gave positive result by M-DDST whereas 27 (87%) were MBL producers by M-CDST. Escherichia coli isolates recorded highest as MR strains were identified. For the treatment, implementation of effective infection control and prevention of nosocomial dissemination used the procedure for detection and identification of carbapenem resistant by most reliable method for study of MBLs produced isolates. The more effective method was M-DDST in comparison of other method as M-CDST.

Keywords: β-lactam antibiotics, Carbapenems, Metallo beta lacatamases, Double disc synergy test, Meropenem

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How to cite this article:

Munesh Kumar Sharma, Dakshina Bisht and Shekhar Pal. 2019. Evaluation of Diagnostic Test in Emerging Carbapenem Resistant Gram Negative Bacilli in Patients admitted to Tertiary Care Centre in North India.Int.J.Curr.Microbiol.App.Sci. 8(4): 2339-2346. doi:
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