Heavy metals are hazardous to living things and causes deterioration of the environment. Nowadays, contamination of heavy metals in the soil and water has gradually increased by human activities. This study analyzed soils from two industrial sites in Myanmar and lead tolerant soil bacteria, E.coil and Alcaligenes sp. were isolated, and characterized. MIC value of lead for Alcaligenes sp. was 13 mM and for E. coil isolates was 10 mM, respectively. Those isolates could tolerate fixed concentrations of three antibiotics (each 50 ug/ml of Chloramphenicol, Kanamycin, Tetracycline) together with 5 mM of Pb (NO3)2 and co-existence of both lead tolerance and antibiotic resistance were found. This research might somehow provide information for heavy metal and antibiotic resistant bacteria and those isolates would be good indicators for further bioremediation processes.

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