National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The history of studies on the role of bacterial antigens for asthma go back to about 100 years ag. Since then, numerous researches have long been interested in the roles of bacteria particularly Staphylococcus aureus (S. aureus), in the pathogenesis of asthma but with controversy. It is just until recently that their significant association has come into the spotlight. In this study (for first time in Iraq ) we taking 100 asthmatic patients and 100 apparent healthy control , the result of ELISA and ASO-PCR and RT - PCR found there was significant relationship between Staphylococcal nasal infection with IFNγ polymorphism also found IL-4 , total - IgE and IFNγ were increased while IL- 10 and FOXP3 were decreased , that number of positive cases with S. aureus infection and nasal polyp had pneumonia and that corresponding with a recent advances in metagenomics technologies that proved the S. aureus bacteria not only colonize in the upper airways and skin but may be extend to lower airways particularly in the subjects with asthma. Also in the present study found that the eosinophil count is higher (675±480.2/cmm) in asthmatic patients carrier to S. aureus than non carrier patients (450.1±145). To determine the relationship between asthma and nasal Staphylococcal infection. (100) one hundred asthmatic patients from Hilla specialized center for allergy with 100 apparently healthy age and sex matched subjects as controls. Blood and nasal swap samples were collected. Blood sample divided into two divisions heparinized part for DNA and RNA extraction and other non-heparinized part for serum isolation and Eosinophil counting. Nasal swap for bacterial culturing. After analysis of the results we found significant increasing in IFNγ, IgE and IL-4 but no effect on FOXP3 and IL-10 titers. Also significant increasing in eosinophil count. Nasal Staphylococcal infection had significant relationship with asthma.
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