International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 848-854

Detection of Amp-C Beta Lactamase Enzyme Production among Enterobacteriaceae and Comparison of Different Inducer Substrate Combinations for Detection of Inducible Amp-C
Sindhu Cugati1* and P.R. Lyra2
1Department of Microbiology, Sree Balaji Medical College and Hospital, Chennai, India
2Department of Microbiology, East Point College of Medical Sciences, Bangalore, India
*Corresponding author

AmpC beta-lactamases hydrolyse penicillins, monobactams, cephalosporins and cephamycins. AmpC producers are resistant tobetalactam/betalactamase inhibitor combinations therapeutically. AmpC is generally underreported which leads to therapeutic failures and uncontrolled spread of these resistant strains. Hence, there is an increased need to detect AmpC routinely in the laboratory. To detect AmpC β-lactamase production among Enterobacteriaceae isolated from clinical samples and to compare different inducer substrate combinations for the detection of inducible Amp-C (iAmpC). 100 clinical isolates of Enterobacteriaceae were tested. Constitutive AmpC (cAmpC) detected using inhibitor based method using Cefoxitin (CN) and CN with Phenylboronic acid (PBA). Inducible AmpC detected using disk approximation test using inducers Imipenen (I) and Cefoxitin (CN), and substrates Cefotaxime (CTX), Ceftazidime (CAZ) and Piperacillin-Tazobactum (PT). Various combinations tested were I/PT, I/CTX, I/CAZ, CN/CTX, CN/CAZ. AmpC production was detected in 30% of isolates, 23% were constitutive and 7% were inducible. Commonest AmpC producer was Enterobacter sp with 7(36.84%) and 4(21.05%), followed by E. coli 14(26.92%) and 3(5.76%) constitutive and iAmpC respectively. 2(8.69%) Klebsiella demonstrated only cAmpC. I/PT combination detected all the 7 iAmpC, others I/CTX and I/CAZ detected only 3isolates. Simple disk method of cefoxitin with boronic acid and I/PT combination can be used to detect constitutive and inducible AmpC respectively.

Keywords: β-lactamases, AmpC, Cephalosporinases, Boronic acid, Inducers

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Sindhu Cugati and Lyra, P.R. 2019. Detection of Amp-C Beta Lactamase Enzyme Production among Enterobacteriaceae and Comparision of Different Inducer Substrate Combinations for Detection of Inducible Amp-CInt.J.Curr.Microbiol.App.Sci. 8(4): 848-854. doi:
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