International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 659-671

Amylase Activity of Starch Degrading Bacteria Isolated from Soil
Patel Nimisha1*, Shah Moksha1 and A.K. Gangawane2
1Parul institute of Applied Science & Research, Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad-380058, Gujarat, India 2Parul institute of Applied science, Parul University, P.O. Limda, Ta. Waghodiya, Dis. Vadodara-390019, Gujarat, India
*Corresponding author

Soil contains huge diversity of microorganism which produces different types of enzymes. Amylase is one of them that hydrolyses starch into its monomer compounds, the smallest being glucose. Hence, amylase is a very prevalent enzyme produced biologically by various kinds of microorganisms and used in industrial sectors for various purposes. Soil bacteria can be isolated and commercially grown in large numbers to produce a vast amount of amylase. In addition, amylases that are extracted require optimum conditions to show greatest activity. In the present study bacteria were isolated from the garden soil and screened for amylase production on starch agar medium. Total 12 isolates were obtained by the primary screening technique from which 05 isolates were showing amylase activity. Zone clearance was determined by Gram’s iodine method. Among 5 isolates isolate 1 was showing highest amylase activity 4.70 U/ml which was considered for further identification. Isolate 1 was tentatively characterized on the basis of their cultural, morphological and biochemical characteristics, which was identified to be Bacillus sp. Further partial purification of the amylase enzyme was carried out by ammonium sulfate precipitation followed by dialysis. Optimization of different parameters was carried out for the amylase production.

Keywords: Amylase, Bacillus subtilis, Soil bacteria

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How to cite this article:

Patel Nimisha, Shah Moksha and Gangawane, A.K. 2019. Amylase Activity of Starch Degrading Bacteria Isolated from Soil.Int.J.Curr.Microbiol.App.Sci. 8(4): 659-671. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.