National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Lepidocephalus thermalis forms a potential candidate species for aquaculture, being a native species it also has high market demand with easy domestication procedure. RNA was extracted from the ovarian tissues of Lepidocephalus thermalis by following the TRIZOL reagent method and cDNA was synthesized. The purity of RNA, cDNA and PCR products were checked. The PCR reactions were carried out with gene specific primers of gonadotropin releasing hormone II, gonadotropin releasing hormone III, leutinizing hormone and follicle stimulating hormone genes and the PCR products size of the four genes were 270-bp for gonadotropin releasing hormone II, 250-bp for gonadotropin releasing hormone III, 290-bp for leutinizing hormone and 260-bp for follicle stimulating hormone. The results from present study will serve as a primary data for further research and development in the reproductive genomic aspects of Indian spiny loach (Lepidocephalus thermalis).
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