International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 920-931

Biocatalytic Reduction of Carbonyl Compounds by Actinobacteria from Two Genera of the Micromonosporaceae Family: Actinoplanes and Dactylosporangium
K. Ishihara1*, K. Morita1, Y. Nishimori1, S. Okamoto1, T. Hiramatsu1, A. Ohkawa1, D. Uesugi2, M. Yanagi1, H. Hamada1, N. Masuoka3 and N. Nakajima4
1Department of Life Science, Okayama University of Science, Okayama, Japan
2Department of Research & Development, JO Cosmetics Co., Ltd., Tokyo, Japan
3Department of Research & Development, Institute for Fruit Juice Research in Tsudaka, Co., Ltd., Okayama, Japan
4Department of Nutritional Science, Okayama Prefectural University, Soja, Okayama, Japan
*Corresponding author

We screened 10 Actinoplanes and 14 Dactylosporangium strains to investigate the biocatalytic ability of two genera of the Micromonosporaceae family. Two Actinoplanes strains (A. ferrugineus NBRC15555 and A. missouriensis NBRC102363) exhibited good growth when cultured in 228 and 231 media, as did two Dactylosporangium strains (Dactylosporangium sp. NBRC101297 and Dactylosporangium sp. NBRC101730) when cultured in 227 and 266 media. The stereoselective reduction of various carbonyl compounds using these four strains was therefore investigated. The present study discovered that these strains can reduce aliphatic and aromatic α-keto esters and an aromatic α-keto amide. On the basis of the conversion ratio and stereoselectivity of the alcohols produced, A. ferrugineus NBRC15555 is a potential biocatalyst for the stereoselective reduction of α-keto esters and an aromatic α-keto amide to the corresponding chiral alcohols when cultured in the 227 medium. Our results also suggest that the reduction of ethyl 2-methylacetoacetate by Dactylosporangium sp. NBRC101730 cultured in 227 medium in the presence of D-glucose is useful for the production of chiral ethyl 3-hydroxy-2-methylbutanoate.

Keywords: Biocatalyst, Actinomycete, Stereoselective reduction, Chiral hydroxy ester, Actinoplanes, Dactylosporangium

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Ishihara, K., K. Morita, Y. Nishimori, S. Okamoto, T. Hiramatsu, A. Ohkawa, D. Uesugi, M. Yanagi, H. Hamada, N. Masuoka and Nakajima, N. 2019. Biocatalytic Reduction of Carbonyl Compounds by Actinobacteria from Two Genera of the Micromonosporaceae Family: Actinoplanes and Dactylosporangium.Int.J.Curr.Microbiol.App.Sci. 8(4): 920-931. doi:
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