National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A study on the development of in vitro propagation protocol for newly developed mutants of Carnation (Dianthus caryophyllus L.)” was carried out during 2017-2018. Results reveal that all the mutants responded well to the in vitro propagation, however ‘UHFS Car-4’ exhibited best propagation potential. Calcium Hypochlorite (5%) treatment for 20 min. is suggested for surface sterilization of explants as it gives 100% uncontaminated growing cultures. For initial culture establishment, MS medium supplemented with 2.0 mgl-1 BA proved to be most effective resulting in earliest establishment. Shoot tips were found better than nodal segment explants for multiplication of carnation. High quality shoots along with best multiplication rate could be obtained in medium containing MS + 3.0 mgl-1 BA + 0.1 mgl-1 NAA. Same medium resulted in maximum shoot proliferation (5.13) in ‘UHFS Car-1’ and ‘UHFS Car-18’. In case of ‘UHFS Car-4’ maximum proliferation (5.36) was noted in MS + BA 2.5 mgl-1 + NAA 0.1 mgl-1 whereas in ‘UHFS Car-33’same effect was obtained (5.26) in MS + BA 2.5 mgl-1 + NAA 0.1 mgl-1 + GA3 1.0 mgl-1. Half strength MS medium containing 2.0 mgl-1 NAA and 0.1% activated charcoal was found to be the best for in vitro rooting of multiplied shoots. A medium containing sand, cocopeat and vermicompost (1:1:1, v/v) was found best for hardening of in vitro regenerated shoots resulting in 79.16% survival and maximum growth under field conditions.
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