International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:4, April, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 183-195

Development of in vitro Propagation Protocol for Newly Developed Mutants of Carnation (Dianthus caryophyllus L.)
Rishu Doad, Puja Sharma, S.R. Dhiman and Priyanka Sharma
Department of Floriculture and Landscape Architecture Dr YSP University of Horticulture and Forestry Nauni, Solan 173230, India
*Corresponding author

A study on the development of in vitro propagation protocol for newly developed mutants of Carnation (Dianthus caryophyllus L.)” was carried out during 2017-2018. Results reveal that all the mutants responded well to the in vitro propagation, however ‘UHFS Car-4’ exhibited best propagation potential. Calcium Hypochlorite (5%) treatment for 20 min. is suggested for surface sterilization of explants as it gives 100% uncontaminated growing cultures. For initial culture establishment, MS medium supplemented with 2.0 mgl-1 BA proved to be most effective resulting in earliest establishment. Shoot tips were found better than nodal segment explants for multiplication of carnation. High quality shoots along with best multiplication rate could be obtained in medium containing MS + 3.0 mgl-1 BA + 0.1 mgl-1 NAA. Same medium resulted in maximum shoot proliferation (5.13) in ‘UHFS Car-1’ and ‘UHFS Car-18’. In case of ‘UHFS Car-4’ maximum proliferation (5.36) was noted in MS + BA 2.5 mgl-1 + NAA 0.1 mgl-1 whereas in ‘UHFS Car-33’same effect was obtained (5.26) in MS + BA 2.5 mgl-1 + NAA 0.1 mgl-1 + GA3 1.0 mgl-1. Half strength MS medium containing 2.0 mgl-1 NAA and 0.1% activated charcoal was found to be the best for in vitro rooting of multiplied shoots. A medium containing sand, cocopeat and vermicompost (1:1:1, v/v) was found best for hardening of in vitro regenerated shoots resulting in 79.16% survival and maximum growth under field conditions.

Keywords: Carnation, in vitro, regeneration, Shoot tip, Nodal segment, Establishment, Multiplication, rooting

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How to cite this article:

Rishu Doad, Puja Sharma, S.R. Dhiman and Priyanka Sharma. 2019. Development of in vitro Propagation Protocol for Newly Developed Mutants of Carnation (Dianthus caryophyllus L.).Int.J.Curr.Microbiol.App.Sci. 8(4): 183-195. doi:
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