National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In-vitro effect of various growth regulators on propagation of Celastrus paniculatus, a medicinally herbs of India was observed in this study. C. paniculatus has been used in the various ancient traditional system of medicine and having a very poor seed germination percentage. Healthy nodal segments of the herb was used as explants with basic MS medium for shoot initiation and multiplication containing various combinations of different growth regulators. MS full and 1/2MS were used for rooting of plantlets with 25 to 200 mg/l Activated Charcoal (AC). Maximum mean number of initiated plantlets 1.8 ± 0.42 with mean length 4.6 ± 1.34 were found in MS medium treated with lowest concentration of BA, while maximum mean number of multiplied plantlets 21.7 ± 1.25 with mean length 6.8 ± 0.91 were found in MS medium treated with 4.44 µM. Maximum mean number of roods16.2 ± 0.78 with Mean length 9.19 ± 0.68 was observed on 1/2MS medium with 100 mg/l activated charcoal. The rooted plantlets were successfully hardened in 1:1:1 ratio of sand: soil: vermicompost and successfully established in soil.
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