International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:12, December, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 635-643

In vitro Effect of Various Plant Growth Regulator on Micro Propagation of Celastrus paniculatus: An Important Medicinal Plant
Rahul Vijay*, Jyoti Shukla and Rajesh Saxena
Madhya Pradesh Council of Science and Technology, Bhopal (M.P), India
*Corresponding author

In-vitro effect of various growth regulators on propagation of Celastrus paniculatus, a medicinally herbs of India was observed in this study. C. paniculatus has been used in the various ancient traditional system of medicine and having a very poor seed germination percentage. Healthy nodal segments of the herb was used as explants with basic MS medium for shoot initiation and multiplication containing various combinations of different growth regulators. MS full and 1/2MS were used for rooting of plantlets with 25 to 200 mg/l Activated Charcoal (AC). Maximum mean number of initiated plantlets 1.8 ± 0.42 with mean length 4.6 ± 1.34 were found in MS medium treated with lowest concentration of BA, while maximum mean number of multiplied plantlets 21.7 ± 1.25 with mean length 6.8 ± 0.91 were found in MS medium treated with 4.44 µM. Maximum mean number of roods16.2 ± 0.78  with Mean length 9.19 ± 0.68 was observed on 1/2MS medium with 100 mg/l activated charcoal. The rooted plantlets were successfully hardened in 1:1:1 ratio of sand: soil: vermicompost and successfully established in soil.

Keywords: Celastrus paniculatus, Micropropagation, Medicinal Plants, Plant Tissue Culture.

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How to cite this article:

Rahul Vijay, Jyoti Shukla and Rajesh Saxena. 2016. In vitro Effect of Various Plant Growth Regulator on Micro Propagation of Celastrus paniculatus: An Important Medicinal Plant.Int.J.Curr.Microbiol.App.Sci. 5(12): 635-643. doi:
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