International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:12, December, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 620-627

Evaluation of different Staining Techniques in the Diagnosis of Trichomonas vaginalis Infection in Females of Reproductive Age Group
T. Shyamalajanaki, G. Sucilathangam*, G. Velvizhi and C. Revathy
Department of Microbiology, Tirunelveli Medical College, Tirunelveli - 627 011,
*Corresponding author

This prospective study was conducted in Department of Microbiology and Outpatient Department of Obstetrics and Gynaecology at Tirunelveli Medical College Hospital, Tirunelveli, and Tamil Nadu from June’ 2015 and July’ 2015.Out of 95 female patients of reproductive age group (15-45 years),Trichomonas vaginalis infection was detected in 16 patients  (16.8%).Vaginal discharge  was the most prevalent symptoms, found in twenty two patients (23.2%).The highest positivity was found in the age group 25-35 years which represent the group of high sexual activity. Wet mount examination for T. vaginalis was positive in 6.3% of cases, whereas, Giemsa staining and Acridine orange staining were positive in 10.5% and 16.8% of cases respectively. Although wet mount examination is the most commonly used test in routine diagnosis of Trichomonas vaginalis infection, but staining techniques should be used as an additional diagnostic test in order to diagnose cases missed either due to unavailability of immediate microscopic facility or delay in the transport of samples to the laboratory for culture.

Keywords: Trichomonas vaginalis,Vaginal discharge, Giemsa staining, Acridine orange staining.

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How to cite this article:

Shyamalajanaki, T., G. Sucilathangam, G. Velvizhi and Revathy, C. 2016. Evaluation of different Staining Techniques in the Diagnosis of Trichomonas vaginalis Infection in Females of Reproductive Age Group.Int.J.Curr.Microbiol.App.Sci. 5(12): 620-627. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.