International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 5, Issue:12, December, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 614-619

Qualitative Assessment of Allergen Skin Test and In vitro Allergen Specific Immunoglobulin E (IgE) Measurement as a Method of Detection of Allergic Symptoms
K. Goswami1, I. Mazumdar2*, S. Mookherjee3 and P. Pal4
1Department of Biochemistry, Lincoln University College, Kuala Lumpur, Malaysia
2Department of Biochemistry, KPC Medical College, Kolkata, India
3Biochemist, North City Diagnostic Centre, Kolkata, India
4Department of Pharmacology, IIMSR & B C Roy Hospital, Haldia, India
*Corresponding author

The increase in air pollutants affects both mortality and morbidity of the respiratory system. People are exposed to various indoor allergens like dust mite, pests and pet shedding, manifesting as Asthma. The diagnosis of allergy is either by allergen specific skin test (SPT) or in vitro specific allergen mediated Immunoglobulin E (IgE) measurement. Allergen specific IgE is indicated for evaluating allergy as a more authentic and specific diagnostic tool. But this has not yet been explored much till date. The present study assessed qualitatively the importance of both SPT and allergen specific IgE measurement for diagnosis of allergy. This study was conducted upon bronchial asthma patients. Allergy SPTs were carried out using commercially available antigens. The concentrations of allergen specific IgE antibodies to allergens with most positive skin test reaction were determined using the rapid, solid phase enzyme linked immunosorbent assay. Trends of reaction patterns remained similar in both. Regression analysis studies revealed positive correlation between the tests following some allergens. But corresponding correlation in all forms of allergens could not be established. Therefore characterization and standardization for the commercial allergen and specific IgE assay kit were proposed. In conclusion, specific IgE to aeroallergens seemed a more specific tool for detecting allergen specificity, though SPT seemed to be more sensitive.

Keywords: House Dust Mite, Asthma,Skin Prick Test, Immunoglobulin E, Environmental Allergens.

Download this article as Download

How to cite this article:

Goswami, K., I. Mazumdar, S. Mookherjee and Pal, P. 2016. Qualitative Assessment of Allergen Skin Test and In vitro Allergen Specific Immunoglobulin E (IgE) Measurement as a Method of Detection of Allergic SymptomsInt.J.Curr.Microbiol.App.Sci. 5(12): 614-619. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.