National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Vacoulating cytotoxin (vacA) gene is one of the most studied virulent genes of Helicobacter pylori which plays a major role in causing inflammation in the human gastric epithelium. We have studied the rate of vacA genotypes of H.pylori in gastric biopsy samples from patients with upper GIT disorders. Antral biopsies (60 nos.) were collected from patients undergoing upper GIT endoscopy at our hospital. Rapid urease Test and Dilute Carbol Fuchsin staining were used to detect H.pylori from the biopsy tissue. The rate of vacA genotypes were studied by PCR, targeting the intermediate region (vacAi) of the vacA gene. 31 of the 60 biopsies were positive for H.pylori by RUT and DCF. vacAi region of the vacA gene was amplified from 9 (29%) of the H.pylori positive cases. The rate of vacA genotypes determined in symptomatic upper GI disorders is very low. This study reveals that vacA is not the one and only factor for H.pylori induced inflammation and further consequences. Gene diversity and combination of virulent genes and their association with other risk factors should be studied to divulge the role virulent genes in H.pylori.
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