Laboratory experiments on seed morpho-metric, physiological and biochemical tests were carried out at Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur during 2017-18. In the present study 13 accessions were used viz., IC-623126, IC-623129, IC-623127, IC-614611, IC-614612, IC-623128, IC-616039, IC-623130, IC-591033, IC-405214, IC-565022, IC-512325 (procured from NBPGR, New Delhi) and a Local variety (Belumgi, Kalburgi Dist.) to assess the seed morpho-metric characters viz., seed colour, spines on seed (by using Leica microscope), seed length, seed breadth, length/breadth ratio (by using Biovis image analyzer), test weight; physiological tests viz., germination, root length, shoot length, seedling length, seedling dry weight, seedling vigour index I and II, biochemical tests viz., dehydrogenase enzyme activity and alpha amylase activity, using Complete Randomized Design in four replication. The results revealed that, the genotypes showed varied response to biochemical tests, physiological parameters and seed morpho-metric characters. Out of 13 genotypes, in 2 genotypes the seed colour was pale brown and in 11 genotypes the seed colour was field drab colour. Spines were present on 9 genotypes and absent in 3 genotypes. The seed length was highest (7.37mm) in IC-405214 and lowest (5.05mm) in IC-616039. The seed breadth was seen highest (4.95mm) in IC-623126 and lowest (3.62mm) in IC-565022. In IC-512325 the length/breadth ratio was highest (1.81 mm), where as in IC-623126 length/breadth ratio was lowest (1.16mm). It was observed that, the test weight of IC-512325 was highest (4.28g) and lowest (1.10g) in IC-623129. Local variety recorded high germination percentage (83.00%), better seedling length IC-623130 (17.78 cm), higher seedling dry matter (27.60 mg) in Local variety with maximum SVI-I (1302.17) and SVI-II (229.47). The genotype IC-623126 recorded comparatively lower germination percentage (43.00 %), numerically lesser seedling length (9.58 cm) in IC-623128, minimum seedling dry matter (1.15 mg) in IC-614612 with low SVI-I (436.52) in IC-512325 and low SVI-II (54.58) in IC-623126. The highest dehydrogenase enzyme activity was observed in in Local variety (0.576) and lowest in IC-623127 (0.080), with the mean of 0.239. The highest α-amylase activity was recorded in Local variety (18.82 mm) and lowest in IC-623128 (14.05 mm) with mean 16.15 mm. It can be concluded that, among the genotypes studied there exists diversity. The characters studied can be used effectively in formulation of National DUS guidelines in India.

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