National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Variations are genetically induced either in vivo or in vitro growth. The objective of the present investigation was to evaluate the effect of variations under in vitro growth in the Chlorophytum borivilianum an endangered medicinal herb. The chemically defined nutrient medium was act as stimulants to the explant and the growth response of regenerates were assessed. The MS media in combination with different growth regulators & pH (chemical factors) and some of the physical factors (external microenvironments i.e. variations in relative humidity, temp. and light/dark periods) was responsible for variations. The in vitro treatment, including different concentrations of the growth regulators were inducing agent. The long time in culture condition produced the greatest probability to initiate variations in clone lines under controlled environment. Three types of regenerates were recorded i.e. normal, vigorous, and dwarf. These variates were marked on the basis of visual score at the time of rooting. Medium compositions were act as elicitor and responsible for variations during the in-vitro multiplications. Due to impact of medium signal changes were recorded in the clones of the same propagules. The obtained mutant variety through in vitro selection was considered as somaclonals. These variations were recorded by visual scoring in their morphology. The variant shows morphological variations from their parental plant.
Citations |
 |
 |
 |
 |
