International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:2, February, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 1372-1378

Analysis of Genetic Diversity of Ferns of Western Ghats in Mudigere region of Karnataka based on ISSR Markers
Vidyashree1*, S.Y. Chandrashekar1, D.C. Lakshmana Reddy2 and Lavanya Reddy2
1Department of Floriculture and Landscape Architecture, College of Horticulture, Mudigere, Karnataka, India
2Division of Biotechnology, Indian Institute of Horticulture Research, Bengaluru, Karnataka, India
*Corresponding author

In view of the high level of morphological diversity in fern species, assessment of genetic variation among 19 fern species was evaluated using ten inter simple sequence repeat (ISSR) markers. The results suggested that the ISSR markers produced much better reproducible bands and were more efficient in grouping fern species. ISSR fragments generated 29 to 87 bands per primer. A total of 281 polymorphic bands generated 34.48 to 66.66 per cent polymorphism per primer. All germplasm were clearly differentiated by their ISSR fingerprints. The similarity coefficient on ISSR profiles were subjected to UPGMA cluster analysis between the species was within the range from 1.00 to 25 per cent. The dendrogram generated by ISSR markers revealed two major clusters, indicating that fern species have distributed based on frond shape, frond type, type of rhizome, habitat, stipe colour, texture, sori position. This is the first report in ferns genetic diversity estimation and clustering by using ISSRs.

Keywords: Ferns, Molecular markers, Cluster analysis, ISSR, Dendrogram

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How to cite this article:

Vidyashree, Chandrashekar, S.Y., D.C. Lakshmana Reddy and Lavanya Reddy. 2019. Analysis of Genetic Diversity of Ferns of Western Ghats in Mudigere region of Karnataka based on ISSR Markers.Int.J.Curr.Microbiol.App.Sci. 8(2): 1372-1378. doi:
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