International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:2, February, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 941-947

Diagnosis of Bovine Sarcocystosis by Conventional Methods
Rasmita Panda*, Manchukonda Udaya Kumar, G.S. Sreenivasa Murthy and Y.N. Reddy
Department of Veterinary Parasitology, College of Veterinary Science, PVNR TVU, Rajendranagar, Hyderabad-500 030, Telangana, India
*Corresponding author

Sarcocystis species are considered as one of the most prevalent parasites of bovines. Sarcocysts occur either macroscopically or microscopically in the tissues of bovine intermediate hosts. Sarcocystosis being an occult infection, most of the time diagnosis in intermediate host by clinical symptoms is unreliable. In this study, the macrosarcocysts were observed in the infected muscle sample by naked eye examination. Suspected muscle samples were examined by muscle squash technique which showed the presence of microsarcocysts in the muscle tissue and bradyzoites were recovered through muscle digestion technique. The detailed morphology of the recovered bradyzoites was studied by Giemsa, Leishman’s and Diff Quick staining which revealed banana shaped bradyzoites with a clear nucleus towards the posterior end. The trypan blue staining indicated live (colourless) and dead (blue) bradyzoites and the gliding movement of the bradyzoites was recorded.

Keywords: Bovine, Sarcocystis, Bradyzoites, Staining

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How to cite this article:

Rasmita Panda, Manchukonda Udaya Kumar, G.S. Sreenivasa Murthy and Reddy, Y.N. 2019. Diagnosis of Bovine Sarcocystosis by Conventional Methods.Int.J.Curr.Microbiol.App.Sci. 8(2): 941-947. doi:
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