International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:2, February, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 906-914

Determination of Genetic Relationship among Wilt Resistant and Susceptible Varieties of Chickpea by RAPD Markers
D.H. Sarnobat*, C.D. Deokar and K.S. Raghuwanshi
Department of Plant Pathology and Agriculture Microbiology, MPKV, Rahuri, India
*Corresponding author

The molecular evaluation of twelve chickpea (Cicer arietinum L.) varieties (screened for their resistance to fusarium wilt) at the Department of Plant Pathology and Agriculture Microbiology in 2014-15 was conducted to assess the genetic diversity and relationship of chickpea genotypes using RAPD. Twenty five primers of RAPD were used of which 15 primers gave amplification products. A total 349 amplicons were obtained of which 331 amplicons were polymorphic with 93.64 % level of polymorphism was observed. Cluster analysis by RAPD and ISSR markers revealed clear distinct diversity between genotypes. The similarity coefficient ranged from 0.64 to 0.92 showed high genetic variability. Digvijay revealed the highest dissimilarity comparing with the other varieties. JG 62 and Vikas showed more similarity than others varieties. The results showed that RAPD analysis for diversity can provide practical information for the management of genetic resources in chickpea breeding program.

Keywords: Chickpea, RAPD, Genetic diversity, Fusarium wilt

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How to cite this article:

Sarnobat, D.H., C.D. Deokar and Raghuwanshi, K.S. 2019. Determination of Genetic Relationship among Wilt Resistant and Susceptible Varieties of Chickpea by RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 8(2): 906-914. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.