International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:2, February, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 884-889

Effect of Hardening Media and Different Cross Combinations on Leaf Gas Exchange Parameters of in vitro Cultured Citrus Plantlets
Anjali Soni1*, A.K. Dubey1, R.M. Sharma1, O.P. Awasthi1 and Bharadwaj Chellapilla2
1Division of Fruits and Horticultural Technology
2Division of Genetics, ICAR- Indian Agricultural Research Institute, New Delhi- 110012, India
*Corresponding author

The present investigation entitled as “Effect of hardening media and different cross combinations on physiological parameters of in vitro cultured citrus plantlets” was carried out in the Division of Fruits and Horticultural Technology, ICAR-IARI, New Delhi. The experiment consisted of embryo rescued plantlets from cross pollination (control pollination) and open pollination (uncontrolled pollination) and two hardening media HM1(Coco peat: Perlite: Vermiculite, 1:1:1) and HM2 (Coco peat: Perlite: Vermiculite, 2:1:1). The results revealed that the progenies of ALC-2 x Kagzi Kalan had highest values of leaf gas exchange traits like internal CO2 concentration (Ci), transpiration rate (E), stomatal conductance (gs) and photosynthesis rate (A) which was followed by progenies of PusaUdit x KagziKalan. However, it was least in progenies of Troyer citrange (Open pollinated). Considering the hardening media, there was a general trend of the higher leaf gas exchange in the HM1media (Coco peat: Perlite: Vermiculite, 1:1:1) compare to HM2 media (Coco peat: Perlite: Vermiculite, 2:1:1).

Keywords: Hardening media, in vitro, cocopeat and leaf gas exchange

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How to cite this article:

Anjali Soni, A.K. Dubey, R.M. Sharma, O.P. Awasthi and Bharadwaj Chellapilla. 2019. Effect of Hardening Media and Different Cross Combinations on Leaf Gas Exchange Parameters of in vitro Cultured Citrus Plantlets.Int.J.Curr.Microbiol.App.Sci. 8(2): 884-889. doi:
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