International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:12, December, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 56-64

Molecular Characterization of Chitinase (chi18-5) and its Expression in richoderma viride: Role on Nematode Egg Parasitism
Rajinikanth1*, M.S. Rao1, K.V. Pavani2 and R. Umamaheswari1
1Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake post, Bengaluru, India – 560 089;
2Gokaraju Rangaraju Institute of Engineering and Technology, Hyderabad,India – 500 090
*Corresponding author

Studies on molecular mechanism of Trichoderma viride in the pathogenesis action on nematode eggs mostly focus on the transcriptional activity of selected genes. However, studies investigating the exact role of a specific gene induction in T. viride on nematode egg parasitism are scant. The molecular interface between T. viride and Meloidogyne incognita eggs was investigated through the qPCR gene expression. The resultant gene chi18-5 of T. viride played a vital role in the induction of mycosis in the nematode eggs. Real-time PCR revealed that chi18-5 started up regulating at 2 hours post-inoculation (hpi) with maximum expression at 5 hpi, and the up regulation of the gene gradually decreased until 19 hpi. The chitinolytic enzyme chi18-5 plays a major role in egg parasitism in transcriptional activity in T.viride was characterized. Our present study proved that chi18-5 encodes one of the lytic enzymes required by T. viride to parasitise nematode eggs.

Keywords: Biological control, chi18-5,Meloidogyne incognita, Enzyme assay,Trichoderma viride

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How to cite this article:

Rajinikanth, R., M.S. Rao, K.V. Pavani and Umamaheswari, R. 2016. Molecular Characterization of Chitinase (chi18-5) and its Expression in Trichoderma viride: Role on Nematode Egg Parasitism.Int.J.Curr.Microbiol.App.Sci. 5(12): 56-64. doi:
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