National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Salmonella is Gram negative, rod-shaped bacteria S. typhimurium not only infects the humans but also infects the animal and poultry. Phage therapy is a method of treatment in which viruses are used to lyses the bacteria. The aim of this study was growth of bacteriophages against S. typhimurium.26 samples were collected from University of Agriculture Faisalabad out of which 5 samples were from poultry droppings, 5samples were from cow fecal, 6 stool samples were from cat, 5 stool samples were from sheep and horse. When these samples were cultured in Tetrathionate broth 21 samples showed turbidity. These 21 samples were cultured on Salmonella Shigella agar. 18samples showed colorless colonies on SS agar. These colonies were further streaked on Hardy CHROME SS agar after 24h incubation at 37oC large black and clear center colonies appeared on this media. Sewage water contains abundant S.Typhimurium serves as vulnerable and extremely flexible host for the development of phages, so 5 sewage water samples were collected. The plaque caused by the phages varies in size and is characterized by spherical zone. The number of PFU from phage lysate was ranging from 105 ×106pfu/ml.
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