National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Rabies is an endemic, fatal zoonotic disease which is transmitted by the bite of an infected animal. The glycoprotein of rabies virus is most antigenic and immunogenic determinant present in rabies virion and can serve as effective target for development of vaccine. The CVS rabies glycoprotein gene (1575 bp) was cloned into cloning vector pTZ57R/T and confirmed by PCR and Sequencing, further the glycoprotein gene was sub cloned into yeast integrative vector pPICZαA for expression and over expressed by using pPIC9K vector. Linearised recombinant vector containing glycoprotein gene was introduced into Pichia pastoris (GS115 and X-33) by electrophoration and the recombinant yeast clones were identified by PCR analysis. The recombinant protein expressed in yeast was confirmed by SDS-PAGE, ELISA, western blot and over expression was confirmed RT-PCR. These results allow us to conclude that P. pastoris is a convenient system for expression as well as for over expression of CVS rabies glycoprotein through multimeriation.
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