National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Exopolysaccharides (EPS) are high molecular weight polymers consisting of sugar residues which widely vary in structure and function. Many bacteria possess the ability to synthesize and excrete exopolysaccharides. In the present study hundred and twenty bacterial isolates were isolated from oil contaminated sites from in and around Navi Mumbai and Thane districts of Maharashtra. Out of which 19 different isolates were selected to explore the EPS production. These isolates were screened for oil emulsification capacity using Emulsification Index 24 test. The isolates were further tested for EPS production and optimization. The optimization was done for different time durations (24h to 1 week), nitrogen sources (Ammonium nitrate, Sodium nitrate and Ammonium chloride) and pH. Culture 1 with the best EPS producing capacity was identified as Bacillus cereus spp.The culture synthesized 520µg/ml of EPS using 0.5% Sodium nitrate at pH 7 in 48h. The FT-IR study revealed the major bands corresponding to O-H, carboxylic acid and H- bonded groups along with stretching of C-O, alcohol, ether and phenol groups. The presence of EPS on the cell surface was confirmed using Scanning Electron Microscopy.
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