International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:11, November, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 230-239

Evaluation of Phenotypic Methods for Detection of Plasmid-Mediated AmpC β-Lactamases (PMABLs) among Klebsiella pneumoniae
Hala B. Othman and Dalia H. Abd ElHamid*
Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Egypt
*Corresponding author

Enterobacteriaceae producing AmpC have become a major therapeutic challenge, as they may appear susceptible to expanded-spectrum cephalosporins when initially tested and may lead to therapeutic failure thereafter. Thus, a simple and reliable detection for AmpC producers is needed. Our aim was to assess different phenotypic tests for detection of plasmid mediated Amp C β lactamases (PMABLs) in Klebsiella pneumoniae compared to PCR for detection of family specific PMABL genes. Eighty clinical isolates of Klebsiella pneumoniae were screened for AmpC production by cefoxtin (FOX) resistance using disk diffusion test, tested by Cloxacillin Combined Disk Diffusion (CC-DD), Cefoxitin-Cloxacillin Double-Disk Synergy (CC-DDS), Ceftazidime-Imipenem Antagonism Test (CIAT), and Cefoxitin-Cefotaxime (FOX-CTX) Antagonism. PMABLs were detected in 26 (32.5%) out of 80 isolates. 40 (50 %) of 80 isolate were resistant to cefoxitin with (84.6%) sensitivity, 26 (32.5%) were positive CC-DD with (61.5%) sensitivity; 22 (27.5%) were positive (CC-DDS) with (38.5%) sensitivity; 4 (5%) were positive (CIAT) 6 (10%) were positive (FOX- CTX). In conclusion, CC-DD is a simple relatively sensitive and specific test for detection of PMABLs. CIAT and FOX- CTX antagonism tests are simple reliable tests for detecting the induced PMABLs.

Keywords: Plasmid mediated AmpC β lactamases (PMABLs),Modified three dimensional test,Ceftazidime-Imipenem antagonism test (CIAT),
 Cefoxitin-Cefotaxime (FOX-CTX) Antagonism

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How to cite this article:

Hala B. Othman and Dalia H. Abd ElHamid. 2016. Evaluation of Phenotypic Methods for Detection of Plasmid-Mediated AmpC β-Lactamases (PMABLs) among Klebsiella pneumoniae.Int.J.Curr.Microbiol.App.Sci. 5(11): 230-239. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.