The present study have been carried out to determine the Effect of Nanosilver and Antibiotics Separately and in Combination on Pseudomonas aeruginosa Clinical Isolates. There were Forty Pseudomonas aeruginosa were isolated from clinical specimens. The MicroScan WalkAway-96 SI System was used in laboratory Identification and antimicrobial susceptibility testing was performed. Also, the well diffusion method was used to evaluate the antibacterial activity of silver nanoparticles in combination with antibiotics.  All isolates were susceptible to more than MIC of nanosilver compared to a high resistance to multiple classes of antibiotics. The Average of inhibition zone diameters of different concentrations of nanosilver dilutions. The combined effect of the silver nanoparticles with antibiotics was assessed in comparison to antibiotics, with well diffusion method. It was observed that the collective effect of silver nanoparticles with antibiotics was additive. The activity of the three antibiotics: gentamycine. ampicillin, and tetracycline with nanoparticles, against Pseudomonas aeruginosa was shown as percentage enhancement in antibacterial effect. Silver nano particles may provide safe and effective treatment for MDR Pseudomonas aeruginosa infections when combined with antibiotics. More further studies are required for understand synergistic effect of nanosilver combining, Assessment of safety and efficacy of new antibiotic- nanosilver combination.

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