National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Brahmi (Bacopa monnieri L.) belonging to the family Scrophulariaceae is an endangered perennial, glaburous, succulent creeping herb of great medicinal importance. Increased demand of Bacopa based drugs makes natural populations of this herb vulnerable to overexploitation. It is therefore essential to optimize and scale up in vitro multiplication protocols for conservation and multiplication of wild and cultivated germplasm. Nodal segments with axillary buds of an elite Bacopa genotype, were surface sterilized and inoculated to initiate aseptic cultures on 0.8 per cent agar semi-solidified MS medium (4.42 g/l) containing 3 per cent sucrose at pH 5.8. For initiating cultures medium was supplemented with different concentrations of BAP (2.0-4.0 mg/l) and kinetin (1.0-2.5 mg/l). The best results were observed on medium containing 2.5 mg/l BAP and 1.5 mg/l Kinetin wherein 100 per cent bud breakage was observed. The maximum shoot proliferation (10.4 ± 0.50 shoots/explant) and shoot length (10.8±0.30 cm) was observed on the same medium. To achieve more shoot length the medium was supplemented with GA3 (1.0 -2.5 mg/l) and the maximum shoot length (16.6 ± 0.29 cm), number of shoots per explants (128.6±0.54) and average number of leaves per shoot (54.6±0.51) were observed in the medium containing 1.5 mg/l GA3. For initiating rooting, cultures were shifted to both semi-solidified and liquid medium with varying strengths of MS salts as well IBA concentrations (0.5-1.5 mg/l). The maximum numbers of roots/shoot (13.2 ± 0.39) and root length (6.6±0.31 cm) were observed in ½ strength liquid MS medium supplemented with IBA 1.0 mg/l. The rooted plantlets were hardened under controlled conditions along with application of ½ strength liquid medium during first two days of ex-vitro acclimatization and 100 per cent survival was achieved. The tissue culture raised plants were shifted to pots and found to be superior with respect to agronomic traits in comparison to naturally propagated plants.
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