The experiment was conducted from September 2017 to February 2018 at Plant Tissue Culture Laboratory, Department of Fruit science, College of Horticulture and Forestry, Jhalrapatan City, Jhalawar to examine the effect of different combinations of plant growth regulators for in-vitro propagation of strawberry. The nodal segments were cultured on MS medium supplemented with different concentrations of shooting (BA, NAA, GA_3, adenine sulphate and coconut water) hormones for direct regeneration. MS media supplemented with 2 ppm BA+ 0.2 ppm NAA+ 1 ppm adenine sulphate took minimum days for sprouting (8.2 days) with highest sprouting percentage (92.59%) and produced maximum length of shoots per culture (3.06 cm), while MS medium supplemented with 1 ppm BA + 0.1 ppm NAA + 1 ppm adenine sulphate + 150 ml coconut water produced the highest number of leaves (8.93) and maximum number of shoot (5.46) each explant.

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