National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Trichoderma is an important biocontrol agent of several soil borne plant pathogens. Genetic modification using mutagenesis offers the potential for producing improved bio protection is likely to enhance their biocontrol capabilities against soil borne pathogen. Molecular techniques are important analytical tool to characterized genetic variability and diagnosis of microbial population. Molecular variability of Trichoderma mother culture and its mutants was studied by using Random Amplified Polymorphic DNA (RAPD). 20 RAPD primer of OPA series were tested, of which 14 primers produced 72 scorable bands among them 52 bands were polymorphic and level of polymorphism was upto 72%. On the basis of dendrogram, the tested cultures were clearly divided into 2 groups. First group i.e. cluster A included 4 mutants TvM-5, TvM-3, TvM-1 TvM-2 and mother culture TvMC. Second group i.e. cluster B included only one mutant i.e. TvPM-4. In ISSR 6 primers were tested, of which, 6 primer produced 32 scorable bands among which 27 bands were polymorphic and level of polymorphism was upto 84%. Two clusters were observed in Dendrogram i.e. Cluster A includes four mutants TvM-5, TvM-3, TvM-1, TvM-2 and mother culture TvMC and cluster B contained only one mutant i.e. TvPM-4.
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