Dengue fever is an emerging acute arbo-viral illness, affecting tropical and subtropical countries. Timely surveillance of dengue fever as well as vector control have utmost importance. Our study has made an attempt to find out the association of dengue fever with seasonal variations and it’s correlation with thrombocytopenia and haematocrit. This study was conducted in central laboratory section of NRI Medical College, Visakhapatnam, AP, from September 2017 to August 2018. Serum samples collected from suspected dengue fever cases were analysed for markers by using immunochromatographic card test. Selected Blood samples were also tested for platelet count and haematocrit by automation method. Of 2002 samples received, 1641(81.96%) sera were positive for one or more parameters of dengue. 1,032 were positive for NS1 only, 228 positive for IgM only and 381 positive for IgG only. Thrombocytopenia was present in 41.6% of selected cases and raised haematocrit in 48.14%. There is a seasonal increase in the incidence of dengue cases during monsoon period. NS1 antigen detection helps in early diagnosis of dengue fever. Platelet count and haematocrit values are useful in the assessment of severity of dengue fever.

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