Dengue Fever (DF), Dengue hemorrhagic fever (DHF) and Dengue Shock Syndrome (DSS) are topmost public health concerns today, especially in tropical and subtropical countries, mainly involving urban and semi-urban areas. In India, Dengue is hyperendemic (Category A) and is a notifiable infectious disease. Though gaps exist in terms of public health measures and health education among public, microbiological laboratory plays a crucial role in confirmation of dengue infection and estimation of burden of disease. The aim of the study was to investigate and assess the epidemiology and prevalence of dengue infection in patients attending our tertiary care hospital in Hyderabad. A total of 1381 patients of suspected Dengue presented during the study period of January – December 2017 were included in the study. All the samples were processed using Dengue NS1 microlisa, Dengue MAC IgM ELISA and Dengue GAC IgG ELISA. Out of these 1381 samples, 395 were serologically positive; 240 (61%) for NS1 antigen and 82 (21%) for anti-dengue IgM and IgG antibodies. Maximum number of dengue positive serum samples was observed in the month of November 421(31%) followed by October 310 (23%). Out of 395 positive dengue cases, 309 were adult and 86 were of pediatric age group. Majority of positive cases falls under age group of 11-20 years 149(38%) followed by 21-30 years of age group 104 (26%). The lowest age of positive case was 4 months and highest was 71 years. Most common clinical presentation was fever followed by thrombocytopenia and arthralgia. To look for platelet and leucocyte counts, blood samples were drawn for routine hemogram at the time of admission. This was evaluated by fully automated haematology analyser-5 part differential. Thrombocytopenia was defined as platelet counts less than 1,50,000/microliter and confirmed on peripheral smear examination. Leucocyte counts of dengue patients were recorded and in majority of patients they were in normal range (4000-11000 per microliter). From the above study, it is concluded that prompt surveillance and regular awareness are the two major steps to be taken for early detection and prevention of dengue virus infection.

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