National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bovine tropical theileriosis caused by Theileria annulata is an economically important disease of cattle in tropical and subtropical countries. Conventional diagnostic methods are unable to identify the subclinical and carrier status of this disease. Molecular diagnostic methods are more sensitive in detection of subclinical and carrier condition on the disease. In this study 817 blood samples were collected from cattle of seven different agroclimatic zone of Tamil Nadu state. DNA extracted from these blood samples were screened by nested Polymerase Chain reaction using primers targeting the partial region of 18SrRNA gene of T. annulata. 114 samples were positive out of 817 samples. North eastern zone showed more prevalence (20.57 %) followed by North western zone (18.80%) whereas hilly zone (3.23%) showed least prevalence of T. annulata in cattle. Present study shows that the 18S rRNA based molecular screening of T. annulata infection in cattle is useful to assess the prevalence of bovine tropical theileriosis.
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