National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The silk fiber of cocoons of silkworms of both the families consists of two major proteins, fibroin and sericin. The fibrion forms central core and sericin forms envelop. Fibroin and sericin, of mulberry silkworm, Bombyx mori deserve appreciable physioc-chemical properties and therefore, they are now recognized as excellent biomaterials in the field of tissue engineering, biotechnology and therapy. Sericin contributes about 20-30 per cent of total cocoon weight. It is characterized by its high content of serine and 18 amino acids, including essential amino acids. There are different methods of isolation of sericin from silk thread. Solubility, molecular weight, and gelling properties of sericin depend on the method of isolation. It has wide applications in pharmaceuticals and cosmetics such as, wound healing, bioadhesive moisturizing, antiwrinkle and antiaging. The present attempt is concerned with assessment of potential regarding antioxidant activity of Sericin from silk from cocoons of non-mulberry silkworm, Antheraea mylitta and mulberry silkworm, Bombyx mori (L) through the use of hydrogen peroxide induced stress in skin fibroblast cell line culture (AH927). Cells treated with sericin exhibited significant cell viability comparable to that of control group (P ＞0.05). The fibroblasts pre-incubated with sericin of both the species at 100 ng/ml had significantly decreased (P ＜0.01) catalase activity. The oxidative stress through hydrogen peroxide was found responsible for significant increase (P ＜0.01) in the release of enzyme (LDH) in comparison with untreated control. The sericin treatment definitely serving to restore the original membrane integrity of the cells. The aqueous form of silk sericin, from a natural source like silkworm cocoon, serves as ideal antioxidant source and may be used to treat the cancer cells.
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