National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was undertaken to understand the genetic diversity of Chilli found in Manipur using RAPD primers. Twenty (20) primers were screened, out of which 9 primers gave polymorphic bands which were used for further analysis. The polymorphic information content (PIC) values ranged from 0.2 (OPB-15) to 0.45 (OPW-04) with an average of 0.35. The resolving power was found highest in OPW04 with value of 7.44 and lowest in OPA11 (2.10). Overall, it was found that OPW04 is a good primer which produced 100% polymorphism with high discriminating power. Cluster analysis using Un-weighted Pair Group Method with Arithmetic Averages (UPGMA) grouped all the chilli samples into three major clusters at a distance coefficient of 0.50, major cluster 1 consist of 4 cultivars (CAU 1, CAU 4, CAU 9, CAU 10). The 2nd major clusters consist of 6 cultivars- (CAU 2, CAU 5, CAU 7, CAU 6, CAU 8, CAU 3. The 3rd major cluster consist of only 1 cultivar (CAU 11).The results showed that RAPD can be effectively used to analysed genetic diversity of chilli found in Manipur.
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