International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:10, October, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 257-262

Genetic Diversity Analysis in Chilli (Capsicum annuum L.) Found in Manipur Using RAPD Markers
Atom Annupama Devi1*, Naorem Brajendra2 and Mutum Dinachandra3
1Department of Plant Molecular Genetics and Genomics, Institute of Bioresources and Sustainable Development, Takyel, Manipur, India
2Department of Genetics and Plant Breeding, Central Agricultural University, CAU, Imphal, India
3Department of Horticulture, Central Agricultural University, CAU, Imphal, India
*Corresponding author

The present study was undertaken to understand the genetic diversity of Chilli found in Manipur using RAPD primers. Twenty (20) primers were screened, out of which 9 primers gave polymorphic bands which were used for further analysis. The polymorphic information content (PIC) values ranged from 0.2 (OPB-15) to 0.45 (OPW-04) with an average of 0.35. The resolving power was found highest in OPW04 with value of 7.44 and lowest in OPA11 (2.10). Overall, it was found that OPW04 is a good primer which produced 100% polymorphism with high discriminating power. Cluster analysis using Un-weighted Pair Group Method with Arithmetic Averages (UPGMA) grouped all the chilli samples into three major clusters at a distance coefficient of 0.50, major cluster 1 consist of 4 cultivars (CAU 1, CAU 4, CAU 9, CAU 10). The 2nd major clusters consist of 6 cultivars- (CAU 2, CAU 5, CAU 7, CAU 6, CAU 8, CAU 3. The 3rd major cluster consist of only 1 cultivar (CAU 11).The results showed that RAPD can be effectively used to analysed genetic diversity of chilli found in Manipur.

Keywords: Genetic diversity, Manipur, polymorphic, RAPD, Polymorphic information content

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How to cite this article:

Atom Annupama Devi, Naorem Brajendra and Mutum Dinachandra. 2018. Genetic Diversity Analysis in Chilli (Capsicum annuum L.) Found in Manipur Using RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 7(10): 257-262. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.