International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:9, September, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 3750-3763

Occurrence of Molds and Identification of Mycoflora Contaminating Millet and Sorghum Produced and Consumed in Benin
Noel Tovide1*, Kifouli Adeoti1, Pacome A. Noumavo1,2, Kamal Garba1, Brice Ohin1, Akim Soninhekpon1, Fidele Tchobo3, Christophe Gandonou4, Fatiou Toukourou1 and Farid Baba-Moussa1
1Laboratoire de Microbiologie et des Technologies Alimentaires, 2Laboratoire de Biologie et de Typage Moleculaire en Microbiologie, 3Laboratoire d'Etude et de Recherche en Chimie Appliquee, 4Laboratoire de Physiologie Vegetale et d'Etude des Stress Environnementaux, Universite deAbomey-Calavi, Benin
*Corresponding author

The purpose of this work is to assess microbial contamination level of millet and sorghum in Benin and to identify the molds responsible for this contamination. Sampling (240 millet and 240 sorghum grain) was done in Beninese agro-ecological zones (I to V) where both cereals are produced. Fungi were isolated using Direct Plating methodand characterised according to macroscopic and microscopic criteria. Grains microbial density was evaluated using the suspension-dilution method. The moisture content obtained per agro-ecological zone varies from 9.12% to 10.42% for millet and from 10.71% to 12.18% for sorghum. Contamination rates range from 37.89% to 52.71% and from 41.89% to 55.11% respectively for millet samples from attics and markets. For sorghum, contamination rates vary from 42.39% to 61.36% for attics samples and from 43.46% to 74.47% for markets samples. The highest total flora was obtained in zone III (40 103 CFU/g) for millet and in zone V (113.25103 CFU/g) for sorghum. With regard to fungal load, the highest values were obtained in zones II and IV (2.5 103 CFU/g) for millet and in zone IV (14.64 103 CFU/g) for sorghum. The 535 molds strains identified mainly belong to the genera Aspergillus (52%) and Penicillium (27%) followed by Fusarium (10%) and Mucor (9%).

Keywords: Millet, Sorghum, Cereals, Contamination, Molds, Aspergillus, Penicillium, Fusarium

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Noel Tovide, Kifouli Adeoti, Pacome A. Noumavo, Kamal Garba, Brice Ohin, Akim Soninhekpon, Fidele Tchobo, Christophe Gandonou, Fatiou Toukourou and Farid Baba-Moussa. 2018. Occurrence of Molds and Identification of Mycoflora Contaminating Millet and Sorghum Produced and Consumed in Benin.Int.J.Curr.Microbiol.App.Sci. 7(9): 3750-3763. doi:
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