National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was aimed to produce a biochemical marker for the medicinally important pteridophytes viz., Marsilea minuta Linn, Ophioglossum petiolatum Hook. and Ampelopteris prolifera (Retz.) Copel. using SDS-PAGE. To produce protein marker for the selected pteridophytes SDS-PAGE was carried out following the method described by Anbalagan. A total of 18 bands with 10 varied Rf values were demonstrated in the SDS-PAGE gel system of pteridophytes. Among the three pteridophytes studied, Marsilea minuta displayed maximum number of protein bands (7) followed by Ampelopteris prolifera (6) and Ophioglossum petiolatum (5). The proteins with Rf vlaues 0.6 and 0.97 showed their unique occurrence in Ampelopteris prolifera. Similarly the proteins with Rf values 0.73 and 0.81 demonstrated their presence only in Marsilea minuta. Ophioglossum petiolatum failed to show its unqueness in the protein gel system of pteridophytes. These banding profiles can be used as biochemical and pharmacognostical marker to distinguish the medicinally important ferns from its adulterants in the pharmaceutical industries.
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