International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 45-48

Molecular characterization of a begomovirus infecting a new host Golden Duranta (Duranta erecta) in India

Avinash Marwal, Anurag Kumar Sahu and R.K.Gaur*

Department of Science, Faculty of Arts, Science and Commerce, Mody Institute of Technology and Science, Lakshmangarh, Sikar-332311, Rajasthan, India *Corresponding author e-mail: gaurrajarshi@hotmail.com

ABSTRACT

In 2012 leaf curl disease was observed on an ornamental plant Golden Duranta (Duranta erecta) in Lakshmangrh, Sikar district of Rajasthan province of India. Affected plants were severely stunted with apical leaf curling and crinkled leaves, symptoms typical of begomovirus infection. An expected PCR product of approximately ~550 bp was amplified, cloned and sequenced. The presence of begomoviruses was also confirmed by Southern blot analysis using cloned DNA-A probe of Papaya leaf curl virus. Sequence analysis of virus infecting Golden Duranta showed 98 % identity with Papaya leaf crumple virus (HE580236). This is the first report of begomovirus associated with leaf curl disease of an ornamental plant Golden Duranta in India.

Keywords

Golden Duranta; Begomovirus; PCR; Southern blot; first report