| International Journal of Current Microbiology and Applied Sciences |
| ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 299-309 |
| Protective effect of flaxseed oil and vitamin E on potassium Bromate-induced oxidative stress in male rats |
| Hala M. A.Wahba1 and Thanaa A.A. Ibrahim2 |
| 1Home Economics Department, Faculty of Specific Education, Minufiya University, Egypt 2Family and Child Sciences Department -Nutrition and Food Science, Faculty of Arts and Sciences, Qassim University, Onaizah, Saudi Arabia *Corresponding author e-mail: hala.ali77@yahoo.com |
| ABSTRACT |
| The protective effect of flaxseed oil (FSO) and vitamin E (Vit E) on potassium bromate (KBrO3)-induced oxidative stress and hepatonephrotoxicity in male rats was investigated. Forty five mature male rats were randomly distributed into 5 groups (n=9). Group (1) was negative control and the other 4 groups were injected with a single dose of KBrO3 (125 mg/kg, i.p.) to induce oxidative stress. Group (2) was used as a positive control, while groups (3), (4) and (5) were orally given FSO (1 ml/rat, 500 mg/kg), Vit E (0.5 ml/rat, 50 mg/kg) and FSO and Vit E in combination, by the same doses daily for 8 weeks respectively. Blood samples were collected for separating the serum for assessment of hepatorenal function. Livers and kidneys were taken for preparing tissue homogenates for biochemical analysis. The results showed that oxidative stress induced by KBrO3 in rats caused significant decreases in body weight gain and feed efficiency ratio. It also significantly increased serum biomarkers of hepatorenal function, increased tissue lipid peroxidation, and decreased the activity of hepatic and renal antioxidant enzymes glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT). Oral coadministration of FSO and Vit E increased body weight gain and feed efficiency ratio. It improved hepatorenal function, reduced tissue lipid peroxidation and normalized the activity of antioxidant enzymes in liver and kidneys of rats given KBrO3. In conclusion, coadministration of FSO and Vit E induces good antioxidant activity and alleviates oxidative stress in rats. The study suggests that intake of flaxseed oil with vitamin E may be beneficial as a food supplement for patients who suffer from oxidative stress and hepatonephrotoxicity |
| Keywords |
| Flaxseed oil; Vitamin E; Oxidative stress; Antioxidants enzymes; Hepatorenal function; Biochemistry; Rats. |