International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 288-298

Exploiting of Streptomyces bottropensis as creator for silver nanoparticles inhibiting growth of microbes

Abdullah Abdullaziz Al-Arfaj*

Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia *Corresponding author e-mail: essam_92003@yahoo.com

ABSTRACT

Synthesis of nanoparticles with interesting physico-chemical properties using efficient as well as eco-friendly technology is one of the main objectives of nanotechnology. In the current study, Streptomyces bottropensis was isolated from soil, Riyadh, Saudi Arabia, and identified by 16S rRNA. Our study showed that shape of silver nanoparticles (AgNPs) was predominantly monodispersed and spherical shapes in the size range of 5 35 nm upon addition of 1 mM silver nitrate. The AgNPs were characterized by determining Fourier transform infrared spectroscopy (FTIR), Energy Dispersive Spectroscopy (EDS) and transmission electron microscopy (TEM). Furthermore, the biosynthesized AgNPs at 5µl significantly inhibited the growth of medically important pathogenic gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes), gram-negative bacteria (Klebsiella pneumoniae and Salmonella typhi) and yeast (Candida albicans). Thus, bioconversion of silver nanoparticles by Streptomyces diastaticuschromogenes could be employed as a potential nanomedicine to eliminate pathogenic microorganisms.

Keywords

AgNPs; Streptomyces bottropensis, silver nanoparticles; Staphylococcus aureus and Streptococcus pyogenes; SEM.