International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 204-214

Molecular diversity analysis of Yersinia enterocolitica isolated from marine marketed fish

S.Akhila1,2, S.Shanmuga Priya1 , T. Senthil Murugn1 and Tha. Thayumanavan1 *

1School of Biotechnology, Dr. G.R. Damodaran College of Science, Coimbatore 641 014. 2Department of Biotechnology, Dhanbagiyam KrishnaswamyMudaliar College for Women, Vellore 632 001. *Corresponding author e-mail: thayumanavan@yahoo.com

ABSTRACT

Of 56 finfish samples collected, 11 of them were contaminated with Y. enterocolitica. Among the different body parts (surface, gill and intestine) of fish tested, maximum incidence has been recorded with gills with 50 %, followed by surface and intestine with 25 % and 8.3% respectively. This indicates that the emergence of pathogenic Y.enterocolitica in marine marketed fish. Multiple antibiotic susceptibility assay showed that all the isolates were resistant to ampicillin and sensitive to chloramphenicol and most of them showed multiple antibiotic resistance. It was also observed that 82 % of the isolates were found to be the producers of haemolysin. The lipopolysaccharide profiles of all the 11 isolate indicates existence of both rough and smooth LPSs. The molecular studies such as RAPD and ERIC PCRs clearly indicated that the wide-genetic diversity among the strains tested.

Keywords

Y. enterocolitica; multiple antibiotic resistances; haemolysin; RAPD-PCR; ERIC-PCR.