International Journal of Current Microbiology and Applied Sciences |
ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 338-346 |
Isolation and characterization of caffeine degrading bacteria
from West Karnataka, India |
J. Sumitha1
* and T. Sivakumar2 |
1Research and Development Centre, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India 1Department of Microbiology, JBAS College for Women, Teynampet, Chennai-600 018, Tamil Nadu, India 2Department of Microbiology, Kanchi Shri Krishna College of Arts and Science, . Kilambi, Kanchipuram-631551, Tamil Nadu, India *Corresponding author e-mail: jsumeetha@gmail.com |
A B S T R A C T |
Isolation of bacteria from soil and leaves of coffee plants and coffee cherries collected in coffee-growing areas of West Karnataka. Thirty two bacterium strains were isolated and exhibited high caffeine tolerance. Pure cultures were maintained
on standard conventional nutrient agar medium.According to the physiological- biochemical characteristics the strains were identified as Pseudomonas and Brevibacterium. The bacterium was characterized with conventional tests and used
to study the tolerance to different concentration of caffeine in both solid and liquid media. Brevibacterium sp. was grown in a liquid minimal medium containing 1- 10g/L caffeine with glucose and sucrose separately. The bacterium was able to
tolerate up to 8g/L of caffeine in solid medium and 3g/L in liquid medium. From
the bacterium, a plasmid of about 2000bp MW was isolated. Pseudomonas sp. tolerated up to 10g/L of caffeine in solid medium and 5g/L in liquid medium. |
Keywords |
Bacteria; caffeine; coffee pulp; Brevibacterium; plasmid. |