| International Journal of Current Microbiology and Applied Sciences |
| ISSN: 2319-7706 Volume 2 Number 8 (2013) pp.206-214 |
| Production, purification and characterization of - amylase using Streptomyces spp. PDS1 and Rhodococcus spp. Isolated from Western Ghats |
| R.Ragunathan1 and R. Padhmadas2 |
| Department of Microbial Biotechnology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore-21, Tamilnadu, India 2Department of Biology, University of Maldives, QAEC, Thinadhoo, Republic of Maldives *Corresponding author e-mail: raguhar@yahoo.co.in |
| A B S T R A C T |
| Streptomyces spp. Pds1 and Rhodococcus spp. were isolated from soil samples collected from Western ghats of Siruvani forest of Tamilnadu. They were used for the production of amylase in the chemically defined medium . Various physico and chemical parameters such as pH, temperature , incubation time were determined. Amylase production was maximum on 3 rd day of incubation. (56U/mL) by Streptomyces spp.pds1 whereas Rhodococcus produced only 50U/Ml on 3 rd day of incubation. The optimum pH and temperature were found to 7.0 for both the strains. The optimum temperature was 40 0C for both the strains . The amylase from the Streptomyces spp. pds1 was partially purified and its molecular weight was determined using SDS- PAGE. The molecular weight was found to be 44kDa. Among the strains tried Streptomyces spp. pds1 was found to be good candidature for amylase production. |
| Keywords |
| Western ghats; amylase; Stroptomyces spp. Pds1; Rhodococcus; Siruvani forest of Tamilnadu optimum activity. |