International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 131-138

In vitro propagation of Bacopa monnieri (L.) Wettst A medicinally priced herb

Jaspreet Kaur, Kalpana Nautiyal and Manu Pant*

Department of Biotechnology, Graphic Era University, Dehradun, India *Corresponding author e-mail: himaniab@gmail.com

A B S T R A C T

Tissue culture studies were performed on Bacopa monnieri (Brahmi). Nodal explants containing preformed axillary bud were used as the starting material. Surface sterilized explants were aseptically cultured on MS medium supplemented with different plant growth regulators. Best results of axillary bud induction were observed on medium containing 1.0 mg/l BAP wherein 100% bud break was achieved. Optimal results of further shoot multiplication were also obtained on same media combination and shoots were periodically subcultured alternately on media containing 0.5 mg/l BAP and basal MS medium without any PGR. Half strength MS media containing different concentrations of auxins were used for in vitro rooting of shoots. Medium supplemented with 1.0 mg/l IBA proved to be most efficient for development of healthy root system. Rooted plantlets were subsequently hardened, acclimatized and successfully established in field with 100% survival rate.

Keywords

Bacopa monneiri; in vitro clonal propagation; medicinal value; field transfer