| International Journal of Current Microbiology and Applied Sciences |
| ISSN: 2319-7706 Volume 2 Number 7 (2013) pp. 44-55 |
| Production of cyclodextrin glycosyl transferase by Bacillus megaterium |
| Nallusamy Sivakumar1 * and Shajahan Shakilabanu2 |
| 1Department of Biology, College of Science, Sultan Qaboos University, PO box: 36, PC 123, Muscat, Oman 2Department of Microbiology, J. J. College of Arts and Science, Pudukkottai, India *Corresponding author e-mail: apnsiva@squ.edu.com |
| A B S T R A C T |
| Cyclodextrin glycosyl transferase (CGTase) fermentation was carried out using a Bacillus megaterium isolated from soil. Different fermentation parameters were tried to optimize the CGTase production. Among the carbon and nitrogen sources tried, maltose was found as the best carbon source and yeast extract was the best nitrogen source for CGTase production. Ca2+ influences the CGTase production and Zn 2+ inhibits the enzyme. High CGTase activity was observed at 24 h of incubation in the medium with initial pH 9 and at temperature 27°C. |
| Keywords |
| Cyclodextrin; CGTase; Bacillus megaterium |