International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 7 (2013) pp. 93-103
Immunomostimulation effects of Sargassum whitti on Mugil cephalus against Pseudomonas fluorescence
S.Kanimozhi, M.Krishnaveni, B.Deivasigmani*, T.Rajasekar and P.Priyadarshni
Department of Biochemistry, Periyar University, Salem-636 011, Tamil Nadu, India Faculty of Marine Sciences, CAS in Marine Biology, Annamalai Univerisity, Parangipettai- 608502, Tamil Nadu, India *Corresponding author e-mail:
The purpose of this study was to determine the effect of water extract of seaweeds Sargassum whitti on the nonspecific immune mechanisms and disease resistance in Mugil cephalus. Sargassum whitti was collected from Pudumadam (Rameshwaram district). The Seaweeds was extracted by using hot water extract method. After extraction it was mix with feed at different concentration. Fishes were feed with different doses of 0.5, 1.0 and 1.5.0 % (kg diet) -1 body weight of water extract of seaweed. The nonspecific immune mechanisms were assessed in terms of Counting of white blood cells (WBC), Lysozyme assay and Respiratory burst activity. The functional immunity in terms of percentage mortality and Relative Percent Survival (RPS) was assessed by a challenge with live P.fluorescence. In our present study 1% Seaweed shows a significantly increased WBC, higher lysozyme activities and respiratory burst activities when compare with other diet 0.5, 1.5% Seaweed died & control feed. Cumulative survival rate after 24h of challenge test, experiment group showed 80% survival and control 70% survival were observed. After 120 h of experimental studies 70%, 80%, 65 %, and 30% survival was observed in G1, G2, G3 and Control group respectively. This preliminary study indicates that Sargassum whitti could be used to promote the health status of fish in intensive finfish aquaculture.
Mugil cephalus; Immunomosti mulation; Sargassum whitti; Pseudomonas fluorescence; non specific immune parameters.