International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 41-56
Identification of genetic elements from A. orientalis contributing to triphenylmethane decolorization
Chengalroyen1* and E.R. Dabbs2
1DST/NRF, Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, University of the Witwatersrand and the National Health Laboratory Service, Johannesburg, South Africa 2Department of Genetics, University of Witwatersrand, Johannesburg, South Africa *Corresponding author e-mail:;
An Amycolatopsis species isolate was found to break down two structurally distinct dyes, one of which is crystal violet. This study aimed to identify the genes involved in triphenylmethane dye biodegradation. A library was constructed and four genes were isolated and identified as3-deoxy-7-phosphoheptulonate synthase, N5, N10- methylenetetrahydro methanopterin reductase, polycystic kidney domain I and glucose/sorbosone dehydrogenase. Gene expression was conducted in the host species Streptomyces lividans. The synergistic action of the segenes led to complete crystal violet decolorization. Additionally, the activity of these genes was tested in two other bacterial species, namely Mycobacterium sp. and Rhodococcus sp. The range of dye classes decolorized was extended in both species, showing that the segenes adopted novel functional potentials within these hosts
Crystal violet; Amycolatopsis sp; decolorization; Mycobacterim sp; Rhodococcus sp.